Journal: Scientific reports

Article Title: Vorinostat attenuates UVB-induced skin senescence by modulating NF-κB and mTOR signaling pathways.

doi: 10.1038/s41598-025-95624-4

Figure Lengend Snippet: Fig. 3. SAHA inhibit UVB-induced inflammatory responses in HaCaT cells. (a) Western blotting of p-p65 and GAPDH protein levels in HaCaT cells treated with UVB irradiation plus 1 µM vorinostat or not on Days 1 and 2. n = 3 biological replicates for each group. (b) Relative protein level ratio of p-p65/GAPDH. (c)–(g) Relative gene expression was normalized to that of GAPDH or 18 s, and the genes IL-1β, IL-6, IL-8, MCP-1 and TNF-α as detected by RT‒qPCR in 48 h. n = 3 biological replicates for each group. The data was expressed as mean ± SD, *p < 0.05, **p < 0.01, and ***p < 0.001. CTL: the control group; UVB: the model group; UVB + SAHA: the treatment group. −: untreated; +: treatment.

Article Snippet: Phospho-P38 MAPK (Thr180/Tyr182) Rabbit mAb (Cat #4511, 1:1500) and Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Cat#3033 1:1500) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Western Blot, Irradiation, Gene Expression, Control